某山区县城学龄前儿童感觉统合失调及其影响因素研究

目的 了解湖北省某山区县城学龄前儿童感觉统合失调(SID)状况及其影响因素,为该地学龄前儿童感觉统合失调的干预提供科学依据。方法 2017年10-11月采用整群抽样,将该县城区划分为东、西、南、北、中5个区,每个区随机抽取1个幼儿园。对抽中的幼儿园3~6岁儿童进行半结构化问卷调查,包括感觉统合能力发展评定量表和基本情况。采用多元Logistic回归模型进行危险因素分析。结果 710名儿童中有396名存在感觉统合失调,患病率为55.8%(轻度30.3%,重度25.5%)。男童和女童相比感觉统合失调差异有统计学意义(χ² =10.688,P<0.05)。在多因素分析中,控制了其他混杂因素后,男童感觉统合失调风险较女童更大(OR=1.636,95%CI:1.177~2.273,P=0.003),膳食情况中吃零食(OR=1.672,95%CI:1.158~2.412,P=0.006)、挑食(OR=1.535,95%CI:1.097~2.147,P=0.012)是SID的危险因素;爱运动是SID的保护因素(OR=0.460,95%CI:0.253~0.836,P=0.011)。随着年龄的增加感觉统合失调的风险越小(OR=0.769,95%CI:0.632~0.934,P=0.008)。结论 本研究显示山区县城学龄前儿童感觉统合失调与性别、年龄、儿童饮食习惯及运动状况相关,应对不同性别、年龄段学龄前儿童采取针对性的防控措施。     

经筋刺法结合运动治疗周围性面神经炎的临床疗效观察

目的：观察经筋针刺方法结合运动疗法对周围性面神经炎的临床疗效。方法：将2014年1月至2015年1月收治的面神经炎患者60例随机分成两组,每组30人,观察组采用经筋针刺方法结合运动疗法,对照组单独采用经筋刺法治疗,比较两组患者的临床疗效和症状改善程度。结果：观察组患者治疗总有效率明显高于对照组患者(p<0.05)；按照面瘫类型分组,观察组所分3组患者治疗总有效率显著高于对照组3组患者(p<0.05)；两组患者在治前期,面神经功能评分接近(p>0.05)；在治疗中期,观察组患者评分明显低于对照组患者(p<0.05)；在治疗结束后,观察组患者评分显著低于对照组患者(p<0.01)；观察组痊愈患者治疗完成所需天数为(27.33±2.71)天,明显少于对照组组的(35±1.62)天(p＜0.01)；治疗后两组患者面部表情,眼裂闭合,鼻唇沟变浅,口角歪斜,额纹消失症状体征改善明显,而观察组改善程度明显高于对照组(p<0.05)。结论：经筋针刺法结合运动疗法对治疗周围性面神经炎具有一定临床应用价值,值得推广使用。     

Aerobic exercise suppresses hepatocellular carcinoma by downregulating dynamin-related protein 1 through PI3K/AKT pathway

ObjectiveExercise, as a common non-drug intervention, is one of several lifestyle choices known to reduce the risk of cancer. Mitochondrial division has been reported to play a key role in the occurrence and transformation of hepatocellular carcinoma (HCC). This study investigated whether exercise could regulate the occurrence and development of HCC through mitosis.MethodsBioinformatics technology was used to analyze the expression level of dynamin-related protein 1 (DRP1), a key protein of mitochondrial division. The effects of DRP1 and DRP1 inhibitor (mdivi-1) on the proliferation and migration of liver cancer cells BEL-7402 were observed using cell counting kit-8, plate colony formation, transwell cell migration, and scratch experiments. Enzyme-linked immunosorbent assay, Western blot and real-time polymerase chain reaction were used to detect the expression of DRP1 and its downstream phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway. A treadmill exercise intervention was tested in a nude mouse human liver cancer subcutaneous tumor model expressing different levels of DRP1. The size and weight of subcutaneous tumors in mice were detected before and after exercise.ResultsThe expression of DRP1 in liver cancer tissues was significantly upregulated compared with normal liver tissues (P < 0.001). The proliferation rate and the migration of BEL-7402 cells in the DRP1 over-expression group were higher than that in the control group. The mdivi-1 group showed an inhibitory effect on the proliferation and migration of BEL-7402 cells at 50 μmol/L. Aerobic exercise was able to inhibit the expression of DRP1 and decrease the size and weight of subcutaneous tumors. Moreover, the expression of phosphorylated PI3K (p-PI3K) and phosphorylated AKT (p-AKT) decreased in the exercise group. However, exercise could not change p-PI3K and p-AKT levels after knocking down DRP1 or using mdivi-1 on subcutaneous tumor.ConclusionAerobic exercise can suppress the development of tumors partially by regulating DRP1 through PI3K/AKT pathway.     

Therapeutic effectiveness of a single exercise session combined with Walk Aide functional electrical stimulation in post-stroke patients:a crossover design study

A growing body of evidence has suggested that the imbalance of epigenetic markers and oxidative stress appears to be involved in the pathophysiology and progression of stroke. Thus, strategies that modulate these biomarkers might be considered targets for neuroprotection and novel therapeutic opportunities for these patients. Physical exercise has been reported to induce changes in these epigenetic markers and improve clinical outcomes in different populations. However, little is reported on this in post-stroke patients. The purpose of this study was to investigate the effect of a single exercise session with Walk Aide functional electrical stimulation(FES) on cognitive performance, clinical functional parameters, oxidative stress and epigenetic modulation in post-stroke individuals. In this crossover design study, 12 post-stroke individuals aged 54–72 years of either sexes were included and subjected to a single session of exercise(45 minutes) without Walk Aide functional electrical stimulation(EXE alone group), followed by another single session of exercise(45 minutes) with Walk Aide functional electrical stimulation(EXE + FES group). The clinical functional outcome measures, cognitive performance and blood collections for biomarker measurements were assessed pre-and post-intervention. After intervention, higher Berg Balance Scale scores were obtained in the EXE + FES group than in the EXE alone group. There was no significant difference in the Timed Up and Go test results post-intervention between EXE alone and EXE + FES groups. After intervention, a better cognitive performance was found in both groups compared with before the intervention. After intervention, the Timed Up and Go test scores were higher in the EXE + FES group than in the EXE alone group. In addition, the intervention induced lower levels of lipid peroxidation. After intervention, carbonyl level was lower, superoxide dismutase activity and superoxide dismutase/catalase activity ratio were higher in the EXE + FES group, compared with the EXE group alone. In each group, both histone deacetylase(HDAC2) and histone acetyltransferase activities were increased after intervention compared with before the intervention. These findings suggest that a single exercise session with Walk Aide FES is more effective on balance ability and cognitive performance compared with conventional exercise alone in post-stroke patients. This is likely to be related to the regulation of oxidative stress markers. The present study was approved by the Research Ethics Committee of the Methodist University Center-IPA(approval No. 2.423.376) on December 7, 2017 and registered in the Brazilian Registry of Clinical Trials—Re BEC(RBR-9 phj2 q) on February 11, 2019.     

早期康复护理和功能锻炼在骨创伤患者术后的应用效果

目的探究早期康复护理和功能锻炼应用于骨创伤患者术后效果。方法选取2018年1月—12月本院收治的60例骨创伤手术患者,随机分成观察组与对照组,各组30例。对照组患者接受常规护理,观察组患者实施术后康复护理及功能锻炼。对比两组护理效果。结果观察组的骨折愈合时长与住院时长都短于对照组,差异有统计学意义(P<0.05)。干预6个月后的,观察组的焦虑评分、抑郁评分、疼痛评分以及总分都优于对照组,差异有统计学意义(P<0.05)。结论针对骨创伤手术患者,应用术后康复护理与功能锻炼,可以有效加快患者骨折愈合速度,减轻患者的关节疼痛,促进尽快恢复关节功能,效果突出,推动患者尽早恢复健康。